Giclée A4 Print - TheDeep

This image depicts the space taken up by cell nuclei, which contain the DNA of blood vessels in the developing zebrafish trunk. To produce this image, data were acquired using a state-of-the-art microscope and the individual cell nuclei extracted using a bespoke segmentation workflow. The 3D spaces were extracted using a technique called Voronoi diagram. Lastly, data were colour-coded for depth. Important to note is that the cell nuclei under investigation are about 10 micrometre in diameter, which is 1/100 of a millimetre. The ligther regions are closer to the viewer, while the darker areas are further away.

Technique: The image was acquired using a Zeiss Z.1 light-sheet fluorescence microscope with a Plan-Apochromat 20×/1.0 Corr nd=1.38 objective and a scientific complementary metal-oxide semiconductor detection unit. Signal was acquired in a stable transgenic line. Image pre-processing, segmentation, Voronoi analysis, and depth-coding were done using Fiji.
Size of canvas: 60 x 60 x 2 cm. Material: Microscopy Science Art Print on Canvas.