Giclée A4 Print - Orientation

This image shows a single plane through the eye of a developing zebrafish after significant processing for abstraction. Using this method, the original data are broken into many small pieces based on the cells occupying space in 3D. The cells studied here are the retinal support cells, called Müller glia, that are important for eye health.

Technique: The image was acquired with the Zeiss LSM 900 AiryScan microscope, using a 40x water-immersion LD C-Apochromat (NA 1.1) objective. Processing was conducted using Fiji. Following image rotation and reduction in 3D, data were segmented, orientations analysed, single planes extracted, and false-colourations applied.